pmscarlet i c1 (Addgene inc)
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pmscarlet i c1
Pmscarlet I C1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmscarlet+c1/pmScarlet-i_C1+(Plasmid+%2385044)/pmc12992094-493-6-7
Average 95 stars, based on 103 article reviews
Pmscarlet I C1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmscarlet+c1/pmScarlet-i_C1+(Plasmid+%2385044)/pmc12992094-493-6-7
Average 95 stars, based on 103 article reviews
pmscarlet i c1 - by Bioz Stars,
2026-09
95/100 stars
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Cloning:Article Title: Antisense oligonucleotide allele-specific targeting of EFEMP1 in a patient-derived model of Doyne honeycomb retinal dystrophy Article Snippet: .. USER cloning technology (NEB, Hitchin, UK) was used according to the manufacturer’s instructions to insert the EFEMP1 open reading frame excluding the stop codon into the mammalian expression vector p3xFLAG-CMV-14 (E7908) from Merck (Sigma-Aldrich, Gillingham, UK) and Expressing:Article Title: Antisense oligonucleotide allele-specific targeting of EFEMP1 in a patient-derived model of Doyne honeycomb retinal dystrophy Article Snippet: .. USER cloning technology (NEB, Hitchin, UK) was used according to the manufacturer’s instructions to insert the EFEMP1 open reading frame excluding the stop codon into the mammalian expression vector p3xFLAG-CMV-14 (E7908) from Merck (Sigma-Aldrich, Gillingham, UK) and Plasmid Preparation:Article Title: Antisense oligonucleotide allele-specific targeting of EFEMP1 in a patient-derived model of Doyne honeycomb retinal dystrophy Article Snippet: .. USER cloning technology (NEB, Hitchin, UK) was used according to the manufacturer’s instructions to insert the EFEMP1 open reading frame excluding the stop codon into the mammalian expression vector p3xFLAG-CMV-14 (E7908) from Merck (Sigma-Aldrich, Gillingham, UK) and Transfection:Article Title: Cyclin-dependent kinase-like 5 (CDKL5) binds to talin and is anchored at the postsynaptic density via direct interaction with PDZ domains Article Snippet: At DIV14, 100,000 cortical neurons per coverslip were transfected with 2 μL of Lipofectamine 2000 (ThermoFisher) and 1.2 μg of DNA following the manufacturer’s recommendations. .. The plasmids used for transfections were as follows: PSD-95-pTagRFP (Addgene, #52671), Clone Assay:Article Title: Cyclin-dependent kinase-like 5 (CDKL5) binds to talin and is anchored at the postsynaptic density via direct interaction with PDZ domains Article Snippet: At DIV14, 100,000 cortical neurons per coverslip were transfected with 2 μL of Lipofectamine 2000 (ThermoFisher) and 1.2 μg of DNA following the manufacturer’s recommendations. .. The plasmids used for transfections were as follows: PSD-95-pTagRFP (Addgene, #52671), Construct:Article Title: Cyclin-dependent kinase-like 5 (CDKL5) binds to talin and is anchored at the postsynaptic density via direct interaction with PDZ domains Article Snippet: At DIV14, 100,000 cortical neurons per coverslip were transfected with 2 μL of Lipofectamine 2000 (ThermoFisher) and 1.2 μg of DNA following the manufacturer’s recommendations. .. The plasmids used for transfections were as follows: PSD-95-pTagRFP (Addgene, #52671), Polymerase Chain Reaction:Article Title: Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labelling and functional imaging Article Snippet: VIS-Fbs to GFP with insertions of mTagBFP2, mTFP1, mWasabi, mNeonGreen, mOrange, LSSmOrange, CyOFP1, mScarlet-I, mScarlet, LSSmScarlet, mNeptune2, mCardinal, PAmCherry or mEos4a were designed by swapping miRFP670nano3 gene with FPs genes in the NIR-Fb plasmid (Addgene no.184675) by NEBuilder HiFi DNA Assembly Kit. .. All oligonucleotide primers for PCR amplification were purchased from Thermo Fisher Scientific ( Supplementary Table 1 ). mTagBFP2, mTFP1, mWasabi, mNeonGreen, mOrange, LSSmOrange, CyOFP1, mScarlet-I, mScarlet, LSSmScarlet, mNeptune2, mCardinal, PAmCherry, and mEos4a were amplified from pBAD-mTagBFP2 (Addgene no.34632), pBAD-mTFP1 (Addgene no.54553), pmWasabi-FAK-5 (Addgene no.56502), pmOrange-N1 (Addgene no.54499), pBAD-LSSmOrange (Addgene no.37129), pKK-CyOFP1-TEV (Addgene no.105782), pmScarlet-I-C1 (Addgene no.85044), Amplification:Article Title: Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labelling and functional imaging Article Snippet: VIS-Fbs to GFP with insertions of mTagBFP2, mTFP1, mWasabi, mNeonGreen, mOrange, LSSmOrange, CyOFP1, mScarlet-I, mScarlet, LSSmScarlet, mNeptune2, mCardinal, PAmCherry or mEos4a were designed by swapping miRFP670nano3 gene with FPs genes in the NIR-Fb plasmid (Addgene no.184675) by NEBuilder HiFi DNA Assembly Kit. .. All oligonucleotide primers for PCR amplification were purchased from Thermo Fisher Scientific ( Supplementary Table 1 ). mTagBFP2, mTFP1, mWasabi, mNeonGreen, mOrange, LSSmOrange, CyOFP1, mScarlet-I, mScarlet, LSSmScarlet, mNeptune2, mCardinal, PAmCherry, and mEos4a were amplified from pBAD-mTagBFP2 (Addgene no.34632), pBAD-mTFP1 (Addgene no.54553), pmWasabi-FAK-5 (Addgene no.56502), pmOrange-N1 (Addgene no.54499), pBAD-LSSmOrange (Addgene no.37129), pKK-CyOFP1-TEV (Addgene no.105782), pmScarlet-I-C1 (Addgene no.85044), |
